Catalog Number - 40008-P30-150

The 40008‑P30‑150 Cogent Bidentate C8 Preparative Column is engineered for high‑capacity purification and demanding preparative HPLC workflows, utilizing 4 µm, 100 Å TYPE‑C™ silica hydride particles to provide unique selectivity, fast equilibration, and exceptional durability.

The Bidentate C8 stationary phase delivers moderate hydrophobic retention with alternative selectivity, making it particularly effective when:

• C18 columns retain compounds too strongly

• Faster elution and recovery are desired

• Working with amine‑containing or polar analytes

• High sample loading and fraction collection are required

The 30 mm internal diameter (ID) supports large injection volumes and high sample mass loading, placing this column in the full preparative / purification range.

Preparative Column Use‑Case Framework

150 mm Length – Maximum Resolution & Purity

• Highest resolving power in the series

• Ideal for complex mixtures and difficult separations

• Best for achieving maximum purity and fraction integrity

• Longer run times and higher pressure vs shorter columns

Flow Rate & System Requirements

30 mm ID preparative columns require specialized HPLC systems

• Typical flow rate range: ~40 – 120 mL/min

• Optimal starting range: ~40 – 70 mL/min for maximum resolution

• Requires:

  • Preparative HPLC system with high‑flow capability

  • Reinforced tubing and high‑capacity pump heads

  • Fraction collector for purification workflows

⚠️ Standard analytical HPLC systems are not suitable for this column size.

Flow rates should be optimized based on:

• Solvent viscosity

• Desired separation resolution

• System pressure limits

• Sample load requirements

Why Choose Bidentate C8 for Preparative Work

Compared to traditional C18 phases, Bidentate C8:

• Reduces excessive retention of hydrophobic compounds

• Enables faster elution and recovery

• Provides different selectivity for polar and ionizable analytes

• Enhances recovery of amines and basic compounds

• Improves efficiency in preparative purification workflows

Cogent TYPE‑C™ Advantages

Silica Hydride Surface Technology

• Reduced silanol activity

• Improved peak shape for basic analytes

• Minimized secondary interactions

Faster Equilibration

• Shorter method cycle times

• Increased throughput in prep workflows

Exceptional Column Durability

• Long operational lifetime

• Resistant to hydrolysis and degradation

• Consistent performance under heavy sample loads

Typical Applications

• Preparative purification of pharmaceuticals

• Isolation of metabolites and synthetic intermediates

• Separation of amine‑containing compounds

• High‑load reversed‑phase chromatography

• Situations where C18 is overly retentive

Custom Order Information

This is a made‑to‑order preparative column. Columns of this size are non‑returnable and cannot be canceled once ordered.

Please contact Customer Service for lead time and system compatibility details.